NOTICE: You are viewing a page of the openwetware wiki. Our "dewikify" feature makes a wiki page appear as a normal web page. In April 2017, this feature will GO AWAY and this URL will redirect to the source URL on our wiki. We're sorry for the inconvenience.

home        research        people        papers        teaching        join us        internal        pictures        contact       


kanamycin selection of transgenic seeds

Selection of transformed Arabidopsis (Kanamycin Selection)

  • Sterilize seeds and stratify them for 2 d at 4°C in the dark
  • Sow seeds on ATS + 50 μg/ml Kanamycin
  • Cultivate seedlings for 10 d at 20°C under LD conditions


Evaluation:

  • transformed seedlings are resistant to Kanamycin and show green and open, expanded cotyledons
  • untransformed seedlings are sensitive to Kanamycin and therefore cotyledons appear yellow and closed